Journal: Cancer Cell International

Article Title: Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells

doi: 10.1186/s12935-016-0379-1

Figure Lengend Snippet: Forced expression of TFF3 in cervical cancer cells modulates the expression of malignant progression-related gene markers. a Determination of endogenous expression of TFF3 protein by Western blot in the cervical cancer cells lines CaSki, SiHa, Me180 and Hela and human non-tumor keratinocyte line HaCaT. b Semi-quantitative RT-PCR analysis was used to assess the mRNA levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3 as described in “ ” section. c Western blot analysis was used to assess the protein levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3. d , e Quantitative PCR analysis quantifying the change in expression of various genes associated with malignant progression in SiHa-TFF3/Hela cells. Change in gene expression is expressed as fold difference, respectively. Fold change values are representative of three independent experiments

Article Snippet: Human cervical cancer cell lines SiHa, CaSki, Hela, Me180 and human non-tumor keratinocyte line HaCaT were obtained from Nanjing KeyGen Biotech Co, Ltd (Nanjing,China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Gene Expression

Journal: Oncology Reports

Article Title: Kindlin-2 suppresses cervical cancer cell migration through AKT/mTOR-mediated autophagy induction

doi: 10.3892/or.2020.7603

Figure Lengend Snippet: Kindlin-2 induces autophagy in cervical cancer cells. (A) Western blot results of kindlin-2 expression in cervical cancer cell lines. (B) Western blot results of kindlin-2 in the indicated cells. (C) Expression levels of p62, LC3, and GAPDH in the indicated cell lines. Cells were cultured in normal medium or EBSS-containing medium for 6 h. (D) Representative fluorescence images of CaSki and SiHa cells transfected with mRFP-GFP-LC3 lentivirus (magnification, ×630). (E) GFP and RFP puncta were counted in 30 cells. *P<0.05. NC, negative control; OE, overexpression; KD, knockdown; GFP, green fluorescent protein; RFP, red fluorescent protein; EBSS, Earle's balanced salts solution.

Article Snippet: The SiHa human cervical cancer cell line was purchased from Shanghai GeneChem Co., Ltd., and CaSki and C-33A human cervical cancer cells were purchased from Zhongqiaoxinzhou Biotech.

Techniques: Western Blot, Expressing, Cell Culture, Fluorescence, Transfection, Negative Control, Over Expression, Knockdown

Journal: Oncology Reports

Article Title: Kindlin-2 suppresses cervical cancer cell migration through AKT/mTOR-mediated autophagy induction

doi: 10.3892/or.2020.7603

Figure Lengend Snippet: Kindlin-2 induces autophagy by inactivating the AKT/mTOR pathway. (A) Protein levels of AKT, p-AKT, mTOR, p-mTOR, and GAPDH in the indicated CaSki and SiHa cells as detected by western blotting. (B) The indicated CaSki cells were treated with SC79 (20 µM) for 24 h and the protein levels of AKT, p-AKT, p62, LC3, and GAPDH were determined by western blotting. (C) The indicated SiHa cells were treated with MK2206 (5 µM) for 24 h and protein levels of AKT, p-AKT, p62, LC3 and GAPDH were detected by western blotting. *P<0.05. NC, negative control; OE, overexpression; KD, knockdown; ns, not significant.

Article Snippet: The SiHa human cervical cancer cell line was purchased from Shanghai GeneChem Co., Ltd., and CaSki and C-33A human cervical cancer cells were purchased from Zhongqiaoxinzhou Biotech.

Techniques: Western Blot, Negative Control, Over Expression, Knockdown

Journal: Oncology Reports

Article Title: Kindlin-2 suppresses cervical cancer cell migration through AKT/mTOR-mediated autophagy induction

doi: 10.3892/or.2020.7603

Figure Lengend Snippet: Kindlin-2 impairs the migration of cervical cancer cells by inducing autophagy. CaSki cells were transfected with kindlin-2 lentivirus or NC lentivirus and then treated with 5 mM 3-MA. SiHa cells were transfected with kindlin-2 RNAi or NC lentivirus and then treated with 20 nM RAPA. Wound healing and Transwell migration assays were performed to detect cell migration under different treatments. (A and B) Representative images (magnification, ×100) and quantification of CaSki cells subjected to the indicated treatments after scratch wounding. (C and D) Representative images (magnification, ×100) and quantification of SiHa cells subjected to the indicated treatments after scratch wounding. The wound area was evaluated using ImageJ software. (E) Representative images (magnification, ×200) and quantification of migrated (F) CaSki and (G) SiHa cells. Migrated cells were counted in five random fields. *P<0.05, **P<0.01, ***P<0.001. NC, negative control; OE, overexpression; KD, knockdown; 3-MA, 3-methyladenine; RAPA, rapamycin.

Article Snippet: The SiHa human cervical cancer cell line was purchased from Shanghai GeneChem Co., Ltd., and CaSki and C-33A human cervical cancer cells were purchased from Zhongqiaoxinzhou Biotech.

Techniques: Migration, Transfection, Software, Negative Control, Over Expression, Knockdown